Chem 128. Lec. 06. Intro to Chemical Biology. DNA Reactivity with Small Molecules (English)


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UCI Chem 128 Introduction to Chemical Biology (Winter 2013)
Lec 06. Introduction to Chemical Biology -- DNA Reactivity with Small Molecules
View the complete course: http://ocw.uci.edu/courses/chem_128_introduction_to_chemical_biology.html
Instructor: Gregory Weiss, Ph.D.


License: Creative Commons BY-NC-SA
Terms of Use: http://ocw.uci.edu/info.
More courses at http://ocw.uci.edu


Description: This video is part of a 18-lecture undergraduate-level course titled "Introduction to Chemical Biology" taught at UC Irvine by Professor Gregory Weiss. Introduction to the basic principles of chemical biology: structures and reactivity; chemical mechanisms of enzyme catalysis; chemistry of signaling, biosynthesis, and metabolic pathways.
Introduction to Chemical Biology (Chem 128) is part of OpenChem: http://ocw.uci.edu/openchem


Recorded on January 24, 2013.


Index of Topics:
Lecture
0:01:13 - Week 3 Overview
0:01:53 - Examples of DNA Intercalators
0:04:21 - How to Measure and Calculate the Strength of DNA Hybridization
0:07:22 - DNA Self-Assembly Based on Sequence
0:07:51 - Hybridization of DNA
0:09:20 - Short Stretches of DNA & RNA Can Also Fold
0:10:31 - DNA is Wound Into Supercoils
0:11:57 - Would the Real DNA Structure Please Stand Up?
0:12:42 - Storing 1.7m of DNA compactly
0:13:44 - Needed: DNA Gyrase to Untangle the DNA
0:15:53 - Bacterial DNA is Stored in Plasmids
0:18:29 - Antibiotics as Selection Markers
0:22:24 - Eukaryotic DNA is Wrapped Around Nucleosomes
0:22:41 - Covalent Modification of Histone Tails
0:23:50 - Modifications to the Lysine Sidechains of Histones
0:27:24 - Trapoxin as a Highly Specific HDCA-1 Inhibitor
0:28:49 - Biological Polynucleotide Synthesis
0:32:07 - DNA Polymerases Require a Template and Lengthen An Existing Strand
0:34:36 - DNA Polymerases Require, Slide 2
0:39:20 - DNA Polymerase and Reverse Transcriptase
0:42:10 - DNA Polymerase: Dream Machine!
0:42:34 - DNA Polymerase: Dream Machine! Slide 2
0:43:04 - DNA Polymerase: Dream Machine! Slide 3
0:43:11 - DNA Polymerase: Dream Machine! Slide 4
0:43:38 - DNA Polymerase for Amplification of PCR
0:46:41 - DNA Polymerase Summary
0:46:57 - RT Inhibitors
0:49:16 - White Board Example
0:50:08 - RT Inhibitors (revisited)
0:52:15 - A Brief History of Chemical DNA Synthesis
0:54:19 - DNA Synthesis by Repeating Cycles
0:54:22 - Chemical DNA Synthesis
0:56:22 - DNA Synthesis on Solid Support
0:57:01 - The DNA Microarray
0:57:22 - Comparing Two States with Microarrays
0:57:25 - DNA MicroArrays Can Monitor Transcription of Thousands of Genes Simultaneously
0:58:53 - The DNA Microarray (revisited)
0:59:04 - Comparing Two States with Microarrays (revisited)
1:00:06 - DNA Microarrays Can Monitor Transcription of Thousands of Genes Simultaneously (revisited)
1:00:54 - Small Molecule Fingerprinting with DNA Microarrays
1:02:09 - FK506 Fingerprinting
1:03:58 - Analysis of DNA sizes by electrophoresis
1:06:06 - Example of DNA Agarose Gels
1:07:00 - Visualizing DNA in Gels
1:07:12 - Separating DNA by Capillary Electrophoresis
1:07:55 - DNA Sequencing Uses DNA Synthesis
1:10:37 - DNA Biotechnology
1:11:19 - Modifying DNA: Random and Directed


Required attribution: Weiss, Gregory Introduction to Chemical Biology 128 (UCI OpenCourseWare: University of California, Irvine),  http://ocw.uci.edu/courses/chem_128_introduction_to_chemical_biology.html. [Access date]. License: Creative Commons Attribution-ShareAlike 3.0 United States License.


Author:
Gregory Alan Weiss
Title:
Professor
Department:
Chemistry
Creative Commons License
Chem 128. Intro to Chemical Biology. Lecture 6: DNA Reactivity with Small Molecules by Gregory Weiss is licensed under a Creative Commons Attribution-ShareAlike 3.0 Unported License.
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